Caslavska, Jitka; Thormann, Wolfgang (2006). ITP in dynamically double-coated fused-silica capillaries. Electrophoresis, 27(23), pp. 4618-30. Weinheim: Wiley-VCH 10.1002/elps.200600346
Full text not available from this repository. (Request a copy)Bidirectional ITP in fused-silica capillaries double-coated with Polybrene and poly-(vinylsulfonate) is a robust approach for analysis of low-molecular-mass compounds. EOF towards the cathode is strong (mobility >4.0 x 10(-8) m(2)/Vs) within the entire pH range investigated (2.40-8.08), dependent on ionic strength and buffer used and, at constant ionic strength, higher at alkaline pH. Electrokinetic separations and transport in such coated capillaries can be described with a dynamic computer model which permits the combined simulation of electrophoresis and electroosmosis in which the EOF is predicted either with a constant (i.e. pH- and ionic strength-independent) or a pH- and ionic strength-dependent electroosmotic mobility. Detector profiles predicted by computer simulation agree qualitatively well with bidirectional isotachopherograms that are monitored with a setup comprising two axial contactless conductivity detectors and a UV absorbance detector. The varying EOF predicted with a pH- and ionic strength-dependent electroosmotic mobility can be regarded as being realistic.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute of Clinical Pharmacology and Visceral Research [discontinued] |
UniBE Contributor: |
Thormann, Wolfgang |
ISSN: |
0173-0835 |
ISBN: |
17136714 |
Publisher: |
Wiley-VCH |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:45 |
Last Modified: |
05 Dec 2022 14:14 |
Publisher DOI: |
10.1002/elps.200600346 |
PubMed ID: |
17136714 |
Web of Science ID: |
000242903000004 |
URI: |
https://boris.unibe.ch/id/eprint/18568 (FactScience: 760) |
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