Forterre, Simone; Raila, Jens; Forterre, Franck; Brunnberg, Leo; Schweigert, Florian J (2006). Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs. Veterinary journal, 171(3), pp. 451-5. Amsterdam: Elsevier 10.1016/j.tvjl.2004.11.017
Full text not available from this repository. (Request a copy)The aim of this study was to investigate differences in concentrations of vitamin A, transthyretin (TTR) and retinol-binding protein (RBP) between plasma and cerebrospinal fluid (CSF) in dogs. RBP was detected using ELISA, and both RBP and TTR by Western blot analysis after separation on SDS-PAGE. Vitamin A was determined by high performance liquid chromatography. RBP and TTR as well as vitamin A were detected in all samples but at substantially lower concentrations in CSF compared to plasma. RBP in dog plasma showed a similar molecular mass to that of humans, whereas canine TTR had a lower molecular mass. Comparison between plasma and CSF showed that both RBP and TTR were of lower molecular mass in CSF. In CSF, RBP and retinol were present at 10-100-fold lower concentrations compared to plasma. Retinyl esters were present only in minute amounts in 5/17 samples. In conclusion, the CSF of dogs compared to humans is significantly different in terms of both quality and quantity of transport proteins for vitamin A.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) |
UniBE Contributor: |
Forterre, Simone, Forterre, Franck |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
1090-0233 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:45 |
Last Modified: |
05 Dec 2022 14:14 |
Publisher DOI: |
10.1016/j.tvjl.2004.11.017 |
PubMed ID: |
16624711 |
Web of Science ID: |
000237560600011 |
URI: |
https://boris.unibe.ch/id/eprint/18841 (FactScience: 1087) |
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