A new microbiopsy system enables rapid preparation of tissue for high-pressure freezing

Vanhecke, Dimitri; Eggli, Peter; Graber, Werner; Studer, Daniel (2006). A new microbiopsy system enables rapid preparation of tissue for high-pressure freezing. Methods in molecular biology, 319, pp. 463-477. Totowa, N.J.: Humana Press

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A microbiopsy system was developed to overcome long sampling times for tissues before they are cryo-fixed by high-pressure freezing. A commercially available biopsy gun was adapted to the needs of small-organ excisions, and biopsy needles were modified to allow small samples (0.6 mm x 1.2 mm x 0.3 mm) to be taken. Specimen platelets with a central slot of the same dimensions as the biopsy are used. A self-made transfer device (in the meantime optimized by Leica-Microsystems [Vienna, Austria]) coordinates the transfer of the excised sample from the biopsy needle into the platelet slot and the subsequent loading in a specimen holder, which is then introduced into a high-pressure freezer (Leica EM PACT; Leica Microsystems, Vienna, Austria). Thirty seconds preparation time is needed from excision until high-pressure freezing. Brain, liver, kidney and muscle excisions of anesthetised rats are shown to be well frozen.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
UniBE Contributor:	Eggli, Peter Slade, Studer, Daniel Franz
ISSN:	1064-3745
ISBN:	16719368
Publisher:	Humana Press
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:46
Last Modified:	02 Mar 2023 23:22
PubMed ID:	16719368
URI:	https://boris.unibe.ch/id/eprint/18939 (FactScience: 1210)