Relative sensitivity factors of inorganic cations in frozen-hydrated standards in secondary ion MS analysis

Dérue, C; Gibouin, D; Lefebvre, F; Studer, D; Thellier, M; Ripoll, C (2006). Relative sensitivity factors of inorganic cations in frozen-hydrated standards in secondary ion MS analysis. Analytical chemistry, 78(8), pp. 2471-7. Washington, D.C.: American Chemical Society 10.1021/ac051518u

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We describe the measurement, at 100 K, of the SIMS relative sensitivity factors (RSFs) of the main physiological cations Na+, K+, Mg2+, and Ca2+ in frozen-hydrated (F-H) ionic solutions. Freezing was performed by either plunge freezing or high-pressure freezing. We also report the measurement of the RSFs in flax fibers, which are a model for ions in the plant cell wall, and in F-H ionic samples, which are a model for ions in the vacuole. RSFs were determined under bombardment with neutral oxygen (FAB) for both the fibers and the F-H samples. We show that referencing to ice-characteristic secondary ions is of little value in determining RSFs and that referencing to K is preferable. The RSFs of Na relative to K and of Ca relative to Mg in F-H samples are similar to their respective values in fiber samples, whereas the RSFs of both Ca and Mg relative to K are lower in fibers than in F-H samples. Our data show that the physical factors important for the determination of the RSFs are not the same in F-H samples and in homogeneous matrixes. Our data show that it is possible to perform a SIMS relative quantification of the cations in frozen-hydrated samples with an accuracy on the order of 15%. Referencing to K permits the quantification of the ionic ratios, even when the absolute concentration of the referencing ion is unknown. This is essential for physiological studies of F-H biological samples.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
UniBE Contributor:	Studer, Daniel Franz
ISSN:	0003-2700
ISBN:	16615752
Publisher:	American Chemical Society
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:49
Last Modified:	05 Dec 2022 14:15
Publisher DOI:	10.1021/ac051518u
PubMed ID:	16615752
Web of Science ID:	000236948300001
URI:	https://boris.unibe.ch/id/eprint/20365 (FactScience: 3653)