Navdaev, A; Clemetson, KJ (2002). Glycoprotein Ib cross-linking/ligation on echicetin-coated surfaces or echicetin-IgMkappa in stirred suspension activates platelets by cytoskeleton modulated calcium release. Journal of biological chemistry, 48(277), pp. 45928-34. Bethesda, Md.: American Society for Biochemistry and Molecular Biology 10.1074/jbc.M206129200
Full text not available from this repository. (Request a copy)Cross-linking platelet GPIb with the snake C-type lectin echicetin provides a specific technique for activation via this receptor. This allows GPIb-dependent mechanisms to be studied without the necessity for shear stress-induced binding of von Willebrand factor or primary alpha(IIb)beta(3) involvement. We already showed that platelets are activated, including tyrosine phosphorylation, by echicetin-IgMkappa-induced GPIb cross-linking. We now investigate the mechanism further and demonstrate that platelets, without modulator reagents, spread directly on an echicetin-coated surface, by a GPIb-specific mechanism, causing exocytosis of alpha-granule markers (P-selectin) and activation of alpha(IIb)beta(3). This spreading requires actin polymerization and release of internal calcium stores but is not dependent on external calcium nor on src family tyrosine kinases. Cross-linking of GPIb complex molecules on platelets, either in suspension or via specific surface attachment, is sufficient to induce platelet activation.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute |
UniBE Contributor: |
Clemetson, Kenneth John |
ISSN: |
0021-9258 |
ISBN: |
12324454 |
Publisher: |
American Society for Biochemistry and Molecular Biology |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:52 |
Last Modified: |
05 Dec 2022 14:16 |
Publisher DOI: |
10.1074/jbc.M206129200 |
PubMed ID: |
12324454 |
Web of Science ID: |
000179529300030 |
URI: |
https://boris.unibe.ch/id/eprint/22165 (FactScience: 32168) |
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