Gerber, Simon D; Solioz, Marc (2007). Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination. Journal of basic microbiology, 47(3), pp. 281-6. Weinheim: Wiley-VCH 10.1002/jobm.200610297
Full text not available from this repository. (Request a copy)Lactococcus lactis IL1403 is a Gram-positive bacterium of great biotechnological interest for food grade applications. Its use is however hampered by the difficulty to efficiently transform this strain. We here describe a detailed, optimized electrotransformation protocol which yields a transformation efficiency of 10(6) cfu/microg of DNA with the two E. coli Gram-positive shuttle vectors pC3 and pVA838. The utility of the protocol was demonstrated by the generation of single- and double-knock-out mutants by homologous recombination.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Center of Regenerative Medicine for Skeletal Tissues [discontinued] 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Center of Regenerative Medicine for Skeletal Tissues [discontinued] 04 Faculty of Medicine > Service Sector > Institute of Clinical Pharmacology and Visceral Research [discontinued] |
UniBE Contributor: |
Gerber, Simon (B), Solioz, Marc |
ISSN: |
0233-111X |
ISBN: |
17518422 |
Publisher: |
Wiley-VCH |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:54 |
Last Modified: |
29 Mar 2023 23:32 |
Publisher DOI: |
10.1002/jobm.200610297 |
PubMed ID: |
17518422 |
Web of Science ID: |
000247297200010 |
URI: |
https://boris.unibe.ch/id/eprint/22929 (FactScience: 37757) |
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