Quantification of global DNA methylation with infrared fluorescence in liver and muscle tissues of differentially fed boars

Braunschweig, M.H. (2009). Quantification of global DNA methylation with infrared fluorescence in liver and muscle tissues of differentially fed boars. Luminescence, 24(4), pp. 213-6. Chichester, UK: Wiley & Sons 10.1002/bio.1098

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Methylation of cytosine residues at CpG sites is involved in various biological processes to control gene regulation and gene expression. Global DNA methylation is changed in different tumors and in cloned animals. Global DNA methylation can be accurately quantified by dot blot analysis with infrared (IR) fluorophores. Methylated lambda DNA was used as model DNA to develop and validate an immunochemical assay with IR fluorescence detection. Two different IR fluorophores were used, one to detect 5-methylcytosine and another to account for DNA loading. A sensitive infrared detection method was established which is suitable for accurate and reproducible quantification of global DNA methylation across a wide dynamic range. This method was subsequently employed to quantify global DNA methylation in liver and in muscle tissues of boars which have received either a control diet or a methyl supplemented diet in an ongoing study. A significant difference in global DNA methylation is indicated in muscle but not in liver tissue between the two groups of boars.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
UniBE Contributor:	Braunschweig, Martin
ISSN:	1522-7235
Publisher:	Wiley & Sons
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:25
Last Modified:	05 Dec 2022 14:26
Publisher DOI:	10.1002/bio.1098
Web of Science ID:	000269240800003
URI:	https://boris.unibe.ch/id/eprint/38291 (FactScience: 220937)