Fernández-Busnadiego, Rubén; Zuber, Benoît; Maurer, Ulrike Elisabeth; Cyrklaff, Marek; Baumeister, Wolfgang; Lucic, Vladan (2010). Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography. Journal of cell biology, 188(1), pp. 145-56. New York, N.Y.: Rockefeller Institute Press 10.1083/jcb.200908082
Full text not available from this repository. (Request a copy)The presynaptic terminal contains a complex network of filaments whose precise organization and functions are not yet understood. The cryoelectron tomography experiments reported in this study indicate that these structures play a prominent role in synaptic vesicle release. Docked synaptic vesicles did not make membrane to membrane contact with the active zone but were instead linked to it by tethers of different length. Our observations are consistent with an exocytosis model in which vesicles are first anchored by long (>5 nm) tethers that give way to multiple short tethers once vesicles enter the readily releasable pool. The formation of short tethers was inhibited by tetanus toxin, indicating that it depends on soluble N-ethyl-maleimide sensitive fusion protein attachment protein receptor complex assembly. Vesicles were extensively interlinked via a set of connectors that underwent profound rearrangements upon synaptic stimulation and okadaic acid treatment, suggesting a role of these connectors in synaptic vesicle mobilization and neurotransmitter release.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy |
UniBE Contributor: |
Zuber, Benoît |
ISSN: |
0021-9525 |
Publisher: |
Rockefeller Institute Press |
Language: |
English |
Submitter: |
Benoît Zuber |
Date Deposited: |
04 Oct 2013 14:15 |
Last Modified: |
05 Dec 2022 14:03 |
Publisher DOI: |
10.1083/jcb.200908082 |
PubMed ID: |
20065095 |
Web of Science ID: |
000273507300014 |
URI: |
https://boris.unibe.ch/id/eprint/4093 (FactScience: 208214) |
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