High myeloperoxidase positive cell infiltration in colorectal cancer is an independent favorable prognostic factor

Droeser, Raoul A.; Hirt, Christian; Eppenberger-Castori, Serenella; Zlobec, Inti; Viehl, Carsten T.; Frey, Daniel M.; Nebiker, Christian A.; Rosso, Raffaele; Zuber, Markus; Amicarella, Francesca; Iezzi, Giandomenica; Sconocchia, Giuseppe; Heberer, Michael; Lugli, Alessandro; Tornillo, Luigi; Oertli, Daniel; Terracciano, Luigi; Spagnoli, Giulio C. (2013). High myeloperoxidase positive cell infiltration in colorectal cancer is an independent favorable prognostic factor. PLoS ONE, 8(5), e64814. Public Library of Science 10.1371/journal.pone.0064814

[img]
Preview
Text (PDF)
fetchObject.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (814kB) | Preview

BACKGROUND

Colorectal cancer (CRC) infiltration by adaptive immune system cells correlates with favorable prognosis. The role of the innate immune system is still debated. Here we addressed the prognostic impact of CRC infiltration by neutrophil granulocytes (NG).

METHODS

A TMA including healthy mucosa and clinically annotated CRC specimens (n = 1491) was stained with MPO and CD15 specific antibodies. MPO+ and CD15+ positive immune cells were counted by three independent observers. Phenotypic profiles of CRC infiltrating MPO+ and CD15+ cells were validated by flow cytometry on cell suspensions derived from enzymatically digested surgical specimens. Survival analysis was performed by splitting randomized data in training and validation subsets.

RESULTS

MPO+ and CD15+ cell infiltration were significantly correlated (p<0.0001; r = 0.76). However, only high density of MPO+ cell infiltration was associated with significantly improved survival in training (P = 0.038) and validation (P = 0.002) sets. In multivariate analysis including T and N stage, vascular invasion, tumor border configuration and microsatellite instability status, MPO+ cell infiltration proved an independent prognostic marker overall (P = 0.004; HR = 0.65; CI:±0.15) and in both training (P = 0.048) and validation (P = 0.036) sets. Flow-cytometry analysis of CRC cell suspensions derived from clinical specimens showed that while MPO+ cells were largely CD15+/CD66b+, sizeable percentages of CD15+ and CD66b+ cells were MPO-.

CONCLUSIONS

High density MPO+ cell infiltration is a novel independent favorable prognostic factor in CRC.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Zlobec, Inti, Lugli, Alessandro

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Andrea Arnold

Date Deposited:

07 Apr 2014 08:16

Last Modified:

05 Dec 2022 14:31

Publisher DOI:

10.1371/journal.pone.0064814

PubMed ID:

23734221

BORIS DOI:

10.7892/boris.45958

URI:

https://boris.unibe.ch/id/eprint/45958

Actions (login required)

Edit item Edit item
Provide Feedback