Penetrating keratoplasty and graft rejection in eight horses

Makra, Zita; Tuboly, Tamás; Bodó, Gabor (2013). Penetrating keratoplasty and graft rejection in eight horses. Acta veterinaria Hungarica, 61(2), pp. 160-174. Akadémiai Kiadó 10.1556/AVet.2013.002

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The aim of this study was to describe long-term follow-up and difference in immune reactions in the tear film following penetrating keratoplasty (PK) in horses when differently preserved corneas were utilised. This report describes for the first time the use of corneal grafts preserved in tissue culture media in equine PK. Eight experimental horses with normal eyes were included and freshly harvested, frozen or preserved corneal grafts were used for the PK. The graft-taking technique and storage, PK surgery, postoperative treatments and complications are described. The mean postoperative follow-up time was 286 days. Tear film samples taken before and periodically after surgery were measured for IgM, IgG and IgA contents by direct ELISA. All grafts were incorporated into the donor horse but were rejected to some degree. The differently harvested corneal grafts healed in the same manner and looked similar. Preoperatively, the clear corneas meant low risk for graft failure, and the fresh or stored tissues provided intact endothelium, although there were no clear graft sites postoperatively. The presence of IgA, IgG and IgM was demonstrated in the tear film from the early postoperative period. IgG levels were lower than IgA or IgM and had a constant baseline in every case, as IgA and IgM had great variability with time and an individual pattern in each eye.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > ISME Equine Clinic Bern > ISME Equine Clinic, Internal medicine
UniBE Contributor:	Bodó, Gabor
Subjects:	600 Technology > 630 Agriculture
ISSN:	0236-6290
Publisher:	Akadémiai Kiadó
Language:	English
Submitter:	Susanne Portner
Date Deposited:	22 Aug 2014 15:06
Last Modified:	05 Dec 2022 14:31
Publisher DOI:	10.1556/AVet.2013.002
PubMed ID:	23661385
URI:	https://boris.unibe.ch/id/eprint/46082