Ryser, Manuel; Künzi, Lisa; Geiser, Marianne; Frenz, Martin; Ricka, Jaro (2013). In situ fiber-optical monitoring of cytosolic calcium in tissue explant cultures. Journal of biophotonics, 8(3), pp. 183-195. Wiley-VCH 10.1002/jbio.201300089
Full text not available from this repository. (Request a copy)We present a fluorescence-lifetime based method for monitoring cell and tissue activity in situ, during cell culturing and in the presence of a strong autofluorescence background. The miniature fiber-optic probes are easily incorporated in the tight space of a cell culture chamber or in an endoscope. As a first application we monitored the cytosolic calcium levels in porcine tracheal explant cultures using the Calcium Green-5N (CG5N) indicator. Despite the simplicity of the optical setup we are able to detect changes of calcium concentration as small as 2.5 nM, with a monitoring time resolution of less than 1 s.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Institute of Applied Physics 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy |
UniBE Contributor: |
Ryser, Manuel, Künzi, Lisa, Geiser, Marianne, Frenz, Martin, Ricka, Jaroslav |
Subjects: |
500 Science > 530 Physics 500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1864-063X |
Publisher: |
Wiley-VCH |
Language: |
English |
Submitter: |
Marianne Geiser Kamber |
Date Deposited: |
02 May 2014 08:36 |
Last Modified: |
05 Dec 2022 14:31 |
Publisher DOI: |
10.1002/jbio.201300089 |
PubMed ID: |
24026906 |
Uncontrolled Keywords: |
Calcium Green-5N autofluorescence cell culture ciliated airway epithelium cytosolic calcium fiber-optic online monitoring ratiometric fluorescence spectroscopy |
URI: |
https://boris.unibe.ch/id/eprint/47763 |
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