Baum, Oliver; Schläppi, Simon; Huber-Abel, Felicitas A; Weichert, Alexander; Hoppeler, Hans; Zakrzewicz, Andreas (2011). The beta-isoform of neuronal nitric oxide synthase (nNOS) lacking the PDZ domain is localized at the sarcolemma. FEBS letters, 585(20), pp. 3219-23. Amsterdam: Elsevier 10.1016/j.febslet.2011.09.016
Full text not available from this repository. (Request a copy)In skeletal muscles, the expression of neuronal NO synthase (nNOS) isoforms is uncharacterized at the protein level. We therefore conducted epitope mapping with anti-peptide-antibodies. Antibodies specific for the nNOS N-terminus recognized the 160-kDa alpha-isoform. In contrast, antibodies against the middle portion or the C-terminus of nNOS bound additionally to the truncated 140-kDa beta-isoform which lacks the PDZ-domain present in the alpha-isoform. All nNOS immunohistochemical reactivity was confined to the sarcolemma. Consistently, immunoblotting disclosed both nNOS-isoforms to be co-enriched in the membrane-associated fractions. The beta-isoform was co-immunoprecipitated with alpha-isoform antibodies in muscle extracts indicating an association of both nNOS-isoforms to direct the beta-variant to the sarcolemma.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy |
UniBE Contributor: |
Baum, Oliver, Hoppeler, Hans-Heinrich |
ISSN: |
0014-5793 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:17 |
Last Modified: |
05 Dec 2022 14:04 |
Publisher DOI: |
10.1016/j.febslet.2011.09.016 |
PubMed ID: |
21945318 |
Web of Science ID: |
000296514100011 |
URI: |
https://boris.unibe.ch/id/eprint/5114 (FactScience: 209832) |