Syring, Claudia; Boss, Renate; Reist, Martin; Bodmer, Michèle; Hummerjohann, J.; Gehrig, P.; Graber, Hans Ulrich (2012). Bovine mastitis: the diagnostic properties of a PCR-based assay to monitor the Staphylococcus aureus genotype B status of a herd, using bulk tank milk. Journal of dairy science, 95(7), pp. 3674-3682. American Dairy Science Association 10.3168/jds.2011-4968
Full text not available from this repository. (Request a copy)Staphylococcus aureus genotype B (GTB) is a contagious mastitis pathogen in cattle, occurring in up to 87% of individuals. Because treatment is generally insufficient, culling is often required, leading to large economic loss in the Swiss dairy industry. As the detection of this pathogen in bulk tank milk (BTM) would greatly facilitate its control, a novel real-time quantitative PCR-based assay for BTM has previously been developed and is now being evaluated for its diagnostic properties at the herd level. Herds were initially classified as to their Staph. aureus GTB status by a reference method. Using BTM and herd pools of single-quarter and 4-quarter milk, the herds were then grouped by the novel assay, and the resulting classifications were compared. A total of 54 dairy herds were evaluated. Using the reference method, 21 herds were found to be GTB positive, whereas 33 were found to be negative. Considering the novel assay using both herd pools, all herds were grouped correctly, resulting in maximal diagnostic sensitivities (100%) and specificities (100%). For BTM samples, diagnostic sensitivities and specificities were 90 and 100%, respectively. Two herds were false negative in BTM, because cows with clinical signs of mastitis were not milked into the tank. Besides its excellent diagnostic properties, the assay is characterized by its low detection level, high efficiency, and its suitability for automation. Using the novel knowledge and assay, eradication of Staph. aureus GTB from a dairy herd may be considered as a realistic goal.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Public Health Institute 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) |
UniBE Contributor: |
Syring, Claudia, Boss, Renate, Reist, Martin, Bodmer, Michèle, Graber, Hans Ulrich |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0022-0302 |
Publisher: |
American Dairy Science Association |
Language: |
English |
Submitter: |
Regula Schatzmann |
Date Deposited: |
28 May 2014 11:36 |
Last Modified: |
05 Dec 2022 14:34 |
Publisher DOI: |
10.3168/jds.2011-4968 |
PubMed ID: |
22720925 |
Uncontrolled Keywords: |
Animals, Bacterial Proteins/genetics, Cattle, DNA, Bacterial/genetics/isolation & purification, Female Genotype Mastitis, Bovine/ diagnosis/microbiology Micrococcal Nuclease/genetics Milk/ microbiology Polymerase Chain Reaction/methods/ veterinary Staphylococcal Infections/genetics/ veterinary Staphylococcus aureus/ genetics |
URI: |
https://boris.unibe.ch/id/eprint/53212 |
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