Blank, Fabian; Gerber, Peter; Rothen-Rutishauser, Barbara; Sakulkhu, Usawadee; Salaklang, Jatuporn; De Peyer, Karin; Gehr, Peter; Nicod, Laurent P; Hofmann, Heinrich; Geiser, Thomas; Petri-Fink, Alke; Von Garnier, Christophe (2011). Biomedical nanoparticles modulate specific CD4+ T cell stimulation by inhibition of antigen processing in dendritic cells. Nanotoxicology, 5(4), pp. 606-21. London: Informa Healthcare 10.3109/17435390.2010.541293
Full text not available from this repository. (Request a copy)Understanding how nanoparticles may affect immune responses is an essential prerequisite to developing novel clinical applications. To investigate nanoparticle-dependent outcomes on immune responses, dendritic cells (DCs) were treated with model biomedical poly(vinylalcohol)-coated super-paramagnetic iron oxide nanoparticles (PVA-SPIONs). PVA-SPIONs uptake by human monocyte-derived DCs (MDDCs) was analyzed by flow cytometry (FACS) and advanced imaging techniques. Viability, activation, function, and stimulatory capacity of MDDCs were assessed by FACS and an in vitro CD4+ T cell assay. PVA-SPION uptake was dose-dependent, decreased by lipopolysaccharide (LPS)-induced MDDC maturation at higher particle concentrations, and was inhibited by cytochalasin D pre-treatment. PVA-SPIONs did not alter surface marker expression (CD80, CD83, CD86, myeloid/plasmacytoid DC markers) or antigen-uptake, but decreased the capacity of MDDCs to process antigen, stimulate CD4+ T cells, and induce cytokines. The decreased antigen processing and CD4+ T cell stimulation capability of MDDCs following PVA-SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy |
UniBE Contributor: |
Gehr, Peter |
ISSN: |
1743-5390 |
Publisher: |
Informa Healthcare |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:19 |
Last Modified: |
05 Dec 2022 14:05 |
Publisher DOI: |
10.3109/17435390.2010.541293 |
PubMed ID: |
21231795 |
Web of Science ID: |
000296633100013 |
URI: |
https://boris.unibe.ch/id/eprint/6377 (FactScience: 211330) |
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