Entwicklung einer PCR zum Nachweis der EAV-Infektion

Brunner, Katharina Rebekka; Santschi, Elisabeth; Gerber, Vinzenz; Burger, Dominik; Zanoni, Reto Giacomo (2014). Entwicklung einer PCR zum Nachweis der EAV-Infektion. Schweizer Archiv für Tierheilkunde, 156(11), pp. 527-538. Huber 10.1024/0036-7281/a000643

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The goal of this work was the development of suitable (real-time) RT-PCR techniques for fast and sensitive diagnosis of EAV and for molecular-epidemiological characterisation of viral strains, as an alternative to virus isolation. To this purpose two conventional RT-PCR methods and one real-time RT-PCR were adapted to detect the broadest possible spectrum of viral strains. Several dilutions with Bucyrus strain showed a 100-fold higher sensitivity of real-time RT-PCR and heminested RT-PCR compared to simple RT-PCR. Making use of 11 cell culture supernatants of different EAV isolates and 7 semen samples of positive stallions, the suitability of the techniques could be shown. Phylogenetic analysis of sequences of the newly analysed samples compared with known sequences indicated that more EAV-lineages exist than presently described.

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