The Role of Cells in Meniscal Guided Tissue Regeneration: A Proof of Concept Study in a Goat Model.

Jülke, Henriette; Mainil-Varlet, Pierre; Jakob, Roland P; Brehm, Walter; Schäfer, Birgit; Nesic, Dobrila (2015). The Role of Cells in Meniscal Guided Tissue Regeneration: A Proof of Concept Study in a Goat Model. Cartilage, 6(1), pp. 20-29. Sage 10.1177/1947603514548213

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OBJECTIVE

Successful repair of defects in the avascular zone of meniscus remains a challenge in orthopedics. This proof of concept study aimed to investigate a guided tissue regeneration approach for treatment of tears in meniscus avascular zone in a goat model.

DESIGN

Full-depth longitudinal tear was created in the avascular zone of the meniscus and sutured. In the two treatment groups, porcine collagen membrane was wrapped around the tear without (CM) or with injection of expanded autologous chondrocytes (CM+cells), whereas in the control group the tear remained only sutured. Gait recovery was evaluated during the entire follow-up period. On explantation at 3 and 6 months, macroscopic gross inspection assessed healing of tears, degradation of collagen membrane, potential signs of inflammation, and osteoarthritic changes. Microscopic histology scoring criteria were developed to evaluate healing of tears, the cellular response, and the inflammatory response.

RESULTS

Gait recovery suggested protective effect of collagen membrane and was supported by macroscopical evaluation where improved tear healing was noted in both treated groups. Histology scoring in CM compared to suture group revealed an increase in tear margins contact, newly formed connective tissue between margins, and cell formations surrounded with new matrix after 3 months yet not maintained after 6 months. In contrast, in the CM+cells group these features were observed after 3 and 6 months.

CONCLUSIONS

A transient, short-term guided tissue regeneration of avascular meniscal tears occurred upon application of collagen membrane, whereas addition of expanded autologous chondrocytes supported more sustainable longer term tear healing.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung
UniBE Contributor:	Nesic, Dobrila
Subjects:	600 Technology > 610 Medicine & health
ISSN:	1947-6035
Publisher:	Sage
Language:	English
Submitter:	Fabian Röthlisberger
Date Deposited:	31 Mar 2016 15:53
Last Modified:	05 Dec 2022 14:53
Publisher DOI:	10.1177/1947603514548213
PubMed ID:	26069707
Uncontrolled Keywords:	animal models; biomaterials; chondrocytes; histology scoring system; meniscus
URI:	https://boris.unibe.ch/id/eprint/77889