Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance.

Schieck, Elise; Lartigue, Carole; Frey, Joachim; Vozza, Nicolas; Hegermann, Jan; Miller, Rachel A; Valguarnera, Ezequiel; Muriuki, Cecilia; Meens, Jochen; Nene, Vish; Naessens, Jan; Weber, Johann; Lowary, Todd L; Vashee, Sanjay; Feldman, Mario F; Jores, Jörg (2016). Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance. Molecular microbiology, 99(1), pp. 55-70. Blackwell Science 10.1111/mmi.13213

[img]
Preview
Text
mmi13213.pdf - Published Version
Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC).

Download (13MB) | Preview

Mycoplasma mycoides subsp. capri (Mmc) and subsp. mycoides (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis and septicaemia. They express galactofuranose residues on their surface, but their role in pathogenesis has not yet been determined. The M. mycoides genomes contain up to several copies of the glf gene, which encodes an enzyme catalysing the last step in the synthesis of galactofuranose. We generated a deletion of the glf gene in a strain of Mmc using genome transplantation and tandem repeat endonuclease coupled cleavage (TREC) with yeast as an intermediary host for the genome editing. As expected, the resulting YCp1.1-Δglf strain did not produce the galactofuranose-containing glycans as shown by immunoblots and immuno-electronmicroscopy employing a galactofuranose specific monoclonal antibody. The mutant lacking galactofuranose exhibited a decreased growth rate and a significantly enhanced adhesion to small ruminant cells. The mutant was also 'leaking' as revealed by a β-galactosidase-based assay employing a membrane impermeable substrate. These findings indicate that galactofuranose-containing polysaccharides conceal adhesins and are important for membrane integrity. Unexpectedly, the mutant strain showed increased serum resistance.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Frey, Joachim, Jores, Jörg

Subjects:

600 Technology > 630 Agriculture

ISSN:

0950-382X

Publisher:

Blackwell Science

Language:

English

Submitter:

Barbara Gautschi-Steffen

Date Deposited:

29 Mar 2016 16:22

Last Modified:

05 Dec 2022 14:53

Publisher DOI:

10.1111/mmi.13213

PubMed ID:

26354009

BORIS DOI:

10.7892/boris.78516

URI:

https://boris.unibe.ch/id/eprint/78516

Actions (login required)

Edit item Edit item
Provide Feedback