Jeckelmann, Jean-Marc; Palacin, Manuel; Fotiadis, Dimitrios (2011). A tool for the qualitative comparison of membrane-embedded and detergent-solubilized membrane protein structures in projection. Journal of structural biology, 173(2), pp. 375-81. San Diego, Calif.: Elsevier 10.1016/j.jsb.2010.09.005
Full text not available from this repository. (Request a copy)The calculation of projection structures (PSs) from Protein Data Bank (PDB)-coordinate files of membrane proteins is not well-established. Reports on such attempts exist but are rare. In addition, the different procedures are barely described and thus difficult if not impossible to reproduce. Here we present a simple, fast and well-documented method for the calculation and visualization of PSs from PDB-coordinate files of membrane proteins: the projection structure visualization (PSV)-method. The PSV-method was successfully validated using the PS of aquaporin-1 (AQP1) from 2D crystals and cryo-transmission electron microscopy, and the PDB-coordinate file of AQP1 determined from 3D crystals and X-ray crystallography. Besides AQP1, which is a relatively rigid protein, we also studied a flexible membrane transport protein, i.e. the L-arginine/agmatine antiporter AdiC. Comparison of PSs calculated from the existing PDB-coordinate files of substrate-free and L-arginine-bound AdiC indicated that conformational changes are detected in projection. Importantly, structural differences were found between the PSV-method calculated PSs of the detergent-solubilized AdiC proteins and the PS from cryo-TEM of membrane-embedded AdiC. These differences are particularly exciting since they may reflect a different conformation of AdiC induced by the lateral pressure in the lipid bilayer.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Jeckelmann, Jean-Marc, Fotiadis, Dimitrios José |
ISSN: |
1047-8477 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:23 |
Last Modified: |
05 Dec 2022 14:06 |
Publisher DOI: |
10.1016/j.jsb.2010.09.005 |
PubMed ID: |
20837147 |
Web of Science ID: |
000286846700020 |
URI: |
https://boris.unibe.ch/id/eprint/8147 (FactScience: 213634) |
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