Kuhn-Nentwig, Lucia; Willems, Jean; Seebeck, Thomas; Shalaby, Tarek; Kaiser, Marcel; Nentwig, Wolfgang (2011). Cupiennin 1a exhibits a remarkably broad, non-stereospecific cytolytic activity on bacteria, protozoan parasites, insects, and human cancer cells. Amino acids, 40(1), pp. 69-76. Wien: Springer Vienna 10.1007/s00726-009-0471-0
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Cupiennin 1a, a cytolytic peptide isolated from the venom of the spider Cupiennius salei, exhibits broad membranolytic activity towards bacteria, trypanosomes, and plasmodia, as well as human blood and cancer cells. In analysing the cytolytic activity of synthesised all-d- and all-l-cupiennin 1a towards pro- and eukaryotic cells, a stereospecific mode of membrane destruction could be excluded. The importance of negatively charged sialic acids on the outer leaflet of erythrocytes for the binding and haemolytic activity of l-cupiennin 1a was demonstrated. Reducing the overall negative charges of erythrocytes by partially removing their sialic acids or by protecting them with tri- or pentalysine results in reduced haemolytic activity of the peptide.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Community Ecology [discontinued] 08 Faculty of Science > Department of Biology > Institute of Cell Biology 08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Kuhn-Nentwig, Lucia Gerda, Willems, Johannes, Seebeck, Thomas, Nentwig, Wolfgang |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 590 Animals (Zoology) 500 Science > 580 Plants (Botany) 500 Science > 540 Chemistry |
ISSN: |
0939-4451 |
Publisher: |
Springer Vienna |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:27 |
Last Modified: |
02 Mar 2023 23:21 |
Publisher DOI: |
10.1007/s00726-009-0471-0 |
PubMed ID: |
20140690 |
BORIS DOI: |
10.7892/boris.9819 |
URI: |
https://boris.unibe.ch/id/eprint/9819 (FactScience: 215613) |
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