Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress

Graewe, Stefanie; Rankin, Kathleen E.; Lehmann, Christine; Deschermeier, Christina; Hecht, Leonie; Froehlke, Ulrike; Stanway, Rebecca R.; Heussler, Volker (2011). Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress. PLoS pathogens, 7(9), e1002224. San Francisco, Calif.: Public Library of Science 10.1371/journal.ppat.1002224

[img]
Preview
Text
journal.ppat.1002224.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (3MB) | Preview

The protozoan parasite Plasmodium is transmitted by female Anopheles mosquitoes and undergoes obligatory development within a parasitophorous vacuole in hepatocytes before it is released into the bloodstream. The transition to the blood stage was previously shown to involve the packaging of exoerythrocytic merozoites into membrane-surrounded vesicles, called merosomes, which are delivered directly into liver sinusoids. However, it was unclear whether the membrane of these merosomes was derived from the parasite membrane, the parasitophorous vacuole membrane or the host cell membrane. This knowledge is required to determine how phagocytes will be directed against merosomes. Here, we fluorescently label the candidate membranes and use live cell imaging to show that the merosome membrane derives from the host cell membrane. We also demonstrate that proteins in the host cell membrane are lost during merozoite liberation from the parasitophorous vacuole. Immediately after the breakdown of the parasitophorous vacuole membrane, the host cell mitochondria begin to degenerate and protein biosynthesis arrests. The intact host cell plasma membrane surrounding merosomes allows Plasmodium to mask itself from the host immune system and bypass the numerous Kupffer cells on its way into the bloodstream. This represents an effective strategy for evading host defenses before establishing a blood stage infection.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Limenitakis, Rebecca and Heussler, Volker

ISSN:

1553-7366

Publisher:

Public Library of Science

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:27

Last Modified:

02 Sep 2015 11:46

Publisher DOI:

10.1371/journal.ppat.1002224

Web of Science ID:

000295409000027

BORIS DOI:

10.7892/boris.10097

URI:

https://boris.unibe.ch/id/eprint/10097 (FactScience: 215936)

Actions (login required)

Edit item Edit item
Provide Feedback