Organelle segregation into Plasmodium liver stage merozoites

Stanway, R. R.; Mueller, N.; Zobiak, B.; Graewe, S.; Froehlke, U.; Zessin, P. J.; Aepfelbacher, M.; Heussler, Volker (2011). Organelle segregation into Plasmodium liver stage merozoites. Cellular microbiology, 13(11), pp. 1768-82. Oxford: Blackwell 10.1111/j.1462-5822.2011.01657.x

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The liver stage of the Plasmodium parasite remains one of the most promising targets for intervention against malaria as it is clinically silent, precedes the symptomatic blood stage and represents a bottleneck in the parasite life cycle. However, many aspects of the development of the parasite during this stage are far from understood. During the liver stage, the parasite undergoes extensive replication, forming tens of thousands of infectious merozoites from each invading sporozoite. This implies a very efficient and accurate process of cytokinesis and thus also of organelle development and segregation. We have generated for the first time Plasmodium berghei double-fluorescent parasite lines, allowing visualization of the apicoplast, mitochondria and nuclei in live liver stage parasites. Using these we have seen that in parallel with nuclear division, the apicoplast and mitochondrion become two extensively branched and intertwining structures. The organelles then undergo impressive morphological and positional changes prior to cell division. To form merozoites, the parasite undergoes cytokinesis and the complex process of organelle development and segregation into the forming daughter merozoites could be analysed in detail using the newly generated transgenic parasites.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria

UniBE Contributor:

Limenitakis, Rebecca and Heussler, Volker

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1462-5814

Publisher:

Blackwell

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:27

Last Modified:

11 May 2016 09:54

Publisher DOI:

10.1111/j.1462-5822.2011.01657.x

Web of Science ID:

000296961800001

URI:

https://boris.unibe.ch/id/eprint/10103 (FactScience: 215942)

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