RNA/DNA co-analysis on aged bloodstains from adhesive tapes used for gunshot residue collection from hands.

Grabmüller, Melanie; Schyma, Christian; Madea, Burkhard; Eichhorst, Tim; Courts, Cornelius (2017). RNA/DNA co-analysis on aged bloodstains from adhesive tapes used for gunshot residue collection from hands. Forensic science, medicine, and pathology, 13(2), pp. 161-169. Springer 10.1007/s12024-017-9864-7

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In cases of firearm related fatalities a systematic investigation at the scene of death is indispensable to differentiate between self-inflicted and homicidal gunshot injuries. A common method to preserve gunshot residues (GSR) is their collection using adhesive tapes. However, the biological material gathered at the same time by the tapes would be of special interest if backspatter, ejected from the entrance wound against the direction of fire, could be detected. In the present study we examined the success rate of co-analysis of RNA and DNA recovered from biological traces sampled with adhesive tapes. The material originated from eight cases of fatal gunshots, taken from the hands of suspects or victims, examined 5 to 19 years ago for GSR. For all types of adhesive tapes tested, quantity and quality of the co-extracted nucleic acids was insufficient for successful DNA profiling, but was sufficient for the detection of blood-specific micro RNA (miRNA). In summary, sampling trace evidence from the hands of persons involved in fatal gunshots with adhesive tapes has a long-term detrimental effect on biological traces.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Legal Medicine
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Medicine

UniBE Contributor:

Schyma, Christian

ISSN:

1556-2891

Publisher:

Springer

Language:

English

Submitter:

Antoinette Angehrn

Date Deposited:

07 Aug 2017 13:30

Last Modified:

07 Aug 2017 13:30

Publisher DOI:

10.1007/s12024-017-9864-7

PubMed ID:

28444618

Uncontrolled Keywords:

Adhesive tapes; Backspatter; Blood specific expression; Gunshot residues; RNA/DNA co-analysis

BORIS DOI:

10.7892/boris.101148

URI:

https://boris.unibe.ch/id/eprint/101148

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