Wood combustion particles induce adverse effects to normal and diseased airway epithelia

Krapf, Manuel; Künzi, Lisa; Allenbach, Sandrine; Bruns, Emily A.; Gavarini, Ilaria; El-Haddad, Imad; Slowik, Jay G.; Prévôt, André S. H.; Drinovec, Luka; Močnik, Griša; Dümbgen, Lutz; Salathe, Matthias; Baumlin, Nathalie; Sioutas, Constantinos; Baltensperger, Urs; Dommen, Josef; Geiser, Marianne (2017). Wood combustion particles induce adverse effects to normal and diseased airway epithelia. Environmental Science Processes & Impacts, 19(4), pp. 538-548. Royal Society of Chemistry 10.1039/C6EM00586A

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Residential wood burning is a major source of poorly characterized, deleterious particulate matter, whose composition and toxicity may vary with wood type, burning condition and photochemical age. The causative link between ambient wood particle constituents and observed adverse health effects is currently lacking. Here we investigate the relationship between chemical properties of primary and atmospherically aged wood combustion particles and acute toxicity in human airway epithelial cells. Emissions from a log wood burner were diluted and injected into a smog chamber for photochemical aging. After concentration-enrichment and removal of oxidizing gases, directly emitted and atmospherically aged particles were deposited on cell cultures at the air-liquid interface for 2 hours in an aerosol deposition chamber mimicking physiological conditions in lungs. Cell models were fully differentiated normal and diseased (cystic fibrosis and asthma) human bronchial epithelia (HBE) and the bronchial epithelial cell line BEAS-2B. Cell responses were assessed at 24 hours after aerosol exposure. Atmospherically relevant doses of wood combustion particles significantly increased cell death in all but the asthma cell model. Expression of oxidative stress markers increased in HBE from all donors. Increased cell death and inflammatory responses could not be assigned to a single chemical fraction of the particles. Exposure to primary and aged wood combustion particles caused adverse effects to airway epithelia, apparently induced by several interacting components.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology
08 Faculty of Science > Department of Mathematics and Statistics > Institute of Mathematical Statistics and Actuarial Science

UniBE Contributor:

Künzi, Lisa; Allenbach, Sandrine; Dümbgen, Lutz and Geiser, Marianne

Subjects:

600 Technology > 610 Medicine & health
500 Science > 510 Mathematics

ISSN:

2050-7887

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Lutz Dümbgen

Date Deposited:

25 Jul 2017 10:01

Last Modified:

25 Jul 2017 10:01

Publisher DOI:

10.1039/C6EM00586A

BORIS DOI:

10.7892/boris.101230

URI:

https://boris.unibe.ch/id/eprint/101230

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