The myometrial contractility during late pregnancy in dairy cows, in vitro

Górriz-Martín, L.; Ulbrich, S.E.; Schmicke, M.; Hirsbrunner, Gabriela; Keller, C.; Yücesoy, N.; Pfarrer, C.; Bollwein, H.; Heppelmann, M. (2017). The myometrial contractility during late pregnancy in dairy cows, in vitro. Animal reproduction science, 181, pp. 130-140. Elsevier 10.1016/j.anireprosci.2017.04.001

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This study aimed to investigate the in vitro contractility of the myometrium and its relationship to the blood concentrations of estradiol-17β (E2β), progesterone (P4), 15-keto-13,14-dihydro-PGF2α (PGFM) and ionised calcium (Ca2+) prior to tissue harvest in 12 healthy Holstein-Friesian cows in late pregnancy. Three circular (CM) and 3 longitudinal myometrial (LM) strips were dissected during a caesarean section and mounted in an organ bath containing modified Krebs solution (KS). The spontaneous contractility was recorded during five 30-min time periods (T1 to T5), after which the strips were exposed to increasing concentrations of oxytocin (OT; 10-10-10-7M), a natural PGF2α-analogue (PGF; 10-7-10-4M) and KS (Cont) for four 30-min time periods (T6 to T9). The variables area under the curve (AUC), mean (MA) and maximal amplitude (maxA) were calculated for each T. The blood P4, E2ß, Ca2+ and PGFM values averaged 4.0±1.7ng/mL, 482.3±63.7 pg/mL, 0.8±0.3 mmol/L and 125.3±63.7pg/mL. The LM strips had greater AUC, MA, and maxA than CM, and OT caused greater AUC and MA in both muscle layers than PGF or control treatment (OT>PGF>Cont). Estradiol-17β correlated with AUC and MA of LM at T1 to T5 (r=0.69; P≤0.05). In conclusion, LM and CM strips have different contractile performance but show enhanced activity when stimulated with OT and less activity after PGF stimulation if compared with Cont. Blood concentrations of E2β may be useful as an indicator of uterine contractile performance in late pregnant cattle.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants

UniBE Contributor:

Hirsbrunner, Gabriela

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0378-4320

Publisher:

Elsevier

Language:

English

Submitter:

Jeannette Glauser

Date Deposited:

18 Sep 2017 09:05

Last Modified:

05 Dec 2022 15:06

Publisher DOI:

10.1016/j.anireprosci.2017.04.001

PubMed ID:

28431863

BORIS DOI:

10.7892/boris.101482

URI:

https://boris.unibe.ch/id/eprint/101482

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