Circulating sterols as predictors of early allograft dysfunction and clinical outcome in patients undergoing liver transplantation.

Ceglarek, Uta; Kresse, Kathleen; Becker, Susen; Fiedler, Martin; Thiery, Joachim; Quante, Markus; Wieland, Robert; Bartels, Michael (2016). Circulating sterols as predictors of early allograft dysfunction and clinical outcome in patients undergoing liver transplantation. Metabolomics, 12(12), p. 182. Springer 10.1007/s11306-016-1129-z

[img]
Preview
Text
11306_2016_Article_1129.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (651kB) | Preview

INTRODUCTION Sensitive and specific assessment of the hepatic graft metabolism after liver transplantation (LTX) is essential for early detection of postoperative dysfunction implying the need for consecutive therapeutic interventions. OBJECTIVES Here, we assessed circulating liver metabolites of the cholesterol pathway, amino acids and acylcarnitines and evaluated their predictive value on early allograft dysfunction (EAD) and clinical outcome in the context of LTX. METHODS The metabolites were quantified in the plasma of 40 liver graft recipients one day pre- and 10 days post-LTX by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Plant sterols as well as cholesterol and its precursors were determined in the free and esterified form; lanosterol in the free form only. Metabolites and esterification ratios were compared to the model for early allograft function scoring (MEAF) which is calculated at day 3 post-LTX from routine parameters defining EAD. RESULTS The hepatic esterification ratio of all sterols, but not amino acids and acylcarnitine concentrations, showed substantial metabolic disturbances post-LTX and correlated to the MEAF. In ROC analysis, the low esterification ratio of β-sitosterol and stigmasterol from day 1 and of the other sterols from day 3 were predictive for a high MEAF, i.e. EAD. Additionally, the ratio of esterified β-sitosterol and free lanosterol were predictive for all days and the esterification ratio of the other sterols at day 3 or 4 post-LTX for 3-month mortality. CONCLUSION Low ratios of circulating esterified sterols are associated with a high risk of EAD and impaired clinical outcome in the early postoperative phase following LTX.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry

UniBE Contributor:

Fiedler, Martin

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1573-3882

Publisher:

Springer

Language:

English

Submitter:

Marie-Christine Müller

Date Deposited:

04 Jun 2018 11:18

Last Modified:

22 Oct 2019 20:25

Publisher DOI:

10.1007/s11306-016-1129-z

PubMed ID:

27840599

Uncontrolled Keywords:

Liver transplantation MEAF Sterol metabolism

BORIS DOI:

10.7892/boris.101620

URI:

https://boris.unibe.ch/id/eprint/101620

Actions (login required)

Edit item Edit item
Provide Feedback