Exploration of the visual system: Part 1: Dissection of the mouse eye for RNA, protein, and histological analyses

Escher, Pascal; Schorderet, D.F. (2011). Exploration of the visual system: Part 1: Dissection of the mouse eye for RNA, protein, and histological analyses. In: Current Protocols in Mouse Biology 1 (pp. 445-462). Wiley 10.1002/9780470942390.mo110129

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Due to the power of genetics, the mouse has become a widely used animal model in vision research. However, its eyeball has an axial length of only about 2 mm. The present protocol describes how to easily dissect the small rodent eye post mortem. This allows collecting different tissues of the eye, i.e., cornea, lens, iris, retina, optic nerve, retinal pigment epithelium (RPE), and sclera. We further describe in detail how to process these eye samples in order to obtain high‐quality RNA for RNA expression profiling studies. Depending on the eye tissue to be analyzed, we present appropriate lysis buffers to prepare total protein lysates for immunoblot and immuno‐precipitation analyses. Fixation, inclusion, embedding, and cryosectioning of the globe for routine histological analyses (HE staining, DAPI staining, immunohistochemistry, in situ hybridization) is further presented. These basic protocols should allow novice investigators to obtain eye tissue samples rapidly for their experiments.

Item Type:

Book Section (Book Chapter)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology

UniBE Contributor:

Escher, Pascal

Subjects:

600 Technology > 610 Medicine & health

Publisher:

Wiley

Language:

English

Submitter:

PD Dr. Pascal Escher

Date Deposited:

10 Jul 2017 13:50

Last Modified:

29 Apr 2018 02:13

Publisher DOI:

10.1002/9780470942390.mo110129

Uncontrolled Keywords:

ophthalmology;visual sciences;retina;cornea;lens;iris;retinal pigment epithelium (RPE)

URI:

https://boris.unibe.ch/id/eprint/101765

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