Rat PPARs: quantitative analysis in adult rat tissues and regulation in fasting and refeeding.

Escher, Pascal; Braissant, O; Basu-Modak, S; Michalik, L; Wahli, W; Desvergne, B (2001). Rat PPARs: quantitative analysis in adult rat tissues and regulation in fasting and refeeding. Endocrinology, 142(10), pp. 4195-4202. Endocrine Society 10.1210/endo.142.10.8458

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PPARs are members of the nuclear hormone receptor superfamily and are primarily involved in lipid metabolism. The expression patterns of all 3 PPAR isotypes in 22 adult rat organs were analyzed by a quantitative ribonuclease protection assay. The data obtained allowed comparison of the expression of each isotype to the others and provided new insight into the less studied PPAR beta (NR1C2) expression and function. This isotype shows a ubiquitous expression pattern and is the most abundant of the three PPARs in all analyzed tissues except adipose tissue. Its expression is especially high in the digestive tract, in addition to kidney, heart, diaphragm, and esophagus. After an overnight fast, PPAR beta mRNA levels are dramatically down-regulated in liver and kidney by up to 80% and are rapidly restored to control levels upon refeeding. This tight nutritional regulation is independent of the circulating glucocorticoid levels and the presence of PPAR alpha, whose activity is markedly up-regulated in the liver and small intestine during fasting. Finally, PPAR gamma 2 mRNA levels are decreased by 50% during fasting in both white and brown adipose tissue. In conclusion, fasting can strongly influence PPAR expression, but in only a few selected tissues.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology

UniBE Contributor:

Escher, Pascal

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0013-7227

Publisher:

Endocrine Society

Language:

English

Submitter:

PD Dr. Pascal Escher

Date Deposited:

11 Jul 2017 07:55

Last Modified:

31 Jul 2017 08:31

Publisher DOI:

10.1210/endo.142.10.8458

PubMed ID:

11564675

BORIS DOI:

10.7892/boris.101768

URI:

https://boris.unibe.ch/id/eprint/101768

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