Coupling ex vivo electroporation of mouse retinas and luciferase reporter assays to assess rod-specific promoter activity.

Boulling, Arnaud; Escher, Pascal (2016). Coupling ex vivo electroporation of mouse retinas and luciferase reporter assays to assess rod-specific promoter activity. Experimental eye research, 148, pp. 79-82. Elsevier 10.1016/j.exer.2016.06.002

[img] Text
1-s2.0-S0014483516301397-main.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (323kB) | Request a copy

Ex vivo electroporation of mouse retinas is an established tool to modulate gene expression and to study cell type-specific gene expression. Here we coupled ex vivo electroporation to luciferase reporter assays to facilitate the study of rod-photoreceptor-specific gene promoters. The activity of the rod-specific proximal bovine rhodopsin promoter was significantly increased in C57BL/6J wild-type retinas at postnatal days 1 and 7 by 3.4-fold and 8.7-fold respectively. In C57BL/6J Nr2e3(rd7/rd7) retinas, where the rod photoreceptor-specific nuclear receptor Nr2e3 is not expressed, a significant increase by 2.5-fold was only observed at postnatal day 7. Cone-specific S-opsin promoter activity was not modulated in C57BL/6J wild-type and Nr2e3(rd7/rd7) retinas. Taken together, we describe an easily implementable protocol to assess rod-specific promoter activity in a physiological context resembling that of the developing postnatal mouse retina.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology

UniBE Contributor:

Escher, Pascal

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0014-4835

Publisher:

Elsevier

Language:

English

Submitter:

PD Dr. Pascal Escher

Date Deposited:

10 Jul 2017 10:00

Last Modified:

31 Jul 2017 08:31

Publisher DOI:

10.1016/j.exer.2016.06.002

PubMed ID:

27268947

Uncontrolled Keywords:

Photoreceptor; Promoter studies; Retinal gene expression; Rod promoter; Transfection

BORIS DOI:

10.7892/boris.101790

URI:

https://boris.unibe.ch/id/eprint/101790

Actions (login required)

Edit item Edit item
Provide Feedback