Barbier de Reuille, Pierre; Robinson, Sarah; Smith, Richard S. (2013). Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem Using MorphoGraphX. In: Žárský, Viktor; Cvrčková, Fatima (eds.) Plant Cell Morphogenesis. Methods and Protocols. Methods in Molecular Biology: Vol. 1080 (pp. 121-134). Totowa, NJ: Springer 10.1007/978-1-62703-643-6_10
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Confocal microscopy is a technique widely used to live-image plant tissue. Cells can be visualized by using fluorescent probes that mark the cell wall or plasma membrane. This enables the confocal microscope to be used as a 3D scanner with submicron precision. Here we present a protocol using the 3D image processing software MorphoGraphX (http://www.MorphoGraphX.org) to extract the surface geometry and cell shapes in the shoot apex. By segmenting cells over consecutive time points, precise growth maps of the shoot apex can be produced. It is also possible to tag a protein of interest with a fluorescent marker and quantify protein expression at the cellular level.
Key words
Item Type: |
Book Section (Book Chapter) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) |
UniBE Contributor: |
Barbier de Reuille, Pierre, Robinson, Sarah Jane, Smith, Richard Simon |
Subjects: |
500 Science > 580 Plants (Botany) |
ISBN: |
978-1-62703-642-9 |
Series: |
Methods in Molecular Biology |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Peter Alfred von Ballmoos-Haas |
Date Deposited: |
30 Jan 2018 08:22 |
Last Modified: |
05 Dec 2022 15:06 |
Publisher DOI: |
10.1007/978-1-62703-643-6_10 |
PubMed ID: |
24132424 |
Uncontrolled Keywords: |
Shoot apex; Confocal microscopy; Image analysis; Segmentation; Watershed; Shape analysis; Expression pattern; 3D visualization |
BORIS DOI: |
10.7892/boris.101813 |
URI: |
https://boris.unibe.ch/id/eprint/101813 |
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