Caloric dose-responsive genes in blood cells differentiate the metabolic status of obese men.

Gille, Doreen; Zangger, Nadine; Soneson, Charlotte; Bütikofer, Ueli; Delorenzi, Mauro; Schwander, Flurina; Kopf-Bolanz, Katrin A; Chollet, Magali; Walther, Barbara; Laederach, Kurt; Vergères, Guy (2017). Caloric dose-responsive genes in blood cells differentiate the metabolic status of obese men. The journal of nutritional biochemistry, 43, pp. 156-165. Elsevier 10.1016/j.jnutbio.2017.02.012

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We have investigated the postprandial transcriptional response of blood cells to increasing caloric doses of a meal challenge to test whether the dynamic response of the human organism to the ingestion of food is dependent on metabolic health. The randomized crossover study included seven normal weight and seven obese men consuming three doses (500/1000/1500 kcal) of a high-fat meal. The blood cell transcriptome was measured before and 2, 4, and 6 h after meal ingestion (168 samples). We applied univariate and multivariate statistics to investigate differentially expressed genes in both study groups. We identified 624 probe sets that were up- or down-regulated after the caloric challenge in a dose-dependent manner. These transcripts were most responsive to the 1500 kcal challenge in the obese group and were associated with postprandial insulin and oxidative phosphorylation. Furthermore, the data revealed a separation of the obese group into individuals whose response was close to the normal weight group and individuals with a transcriptional response indicative of a loss of metabolic flexibility. The molecular signature provided by the postprandial transcriptomic response of blood cells to increasing caloric doses of a high-fat meal challenge may represent a sensitive way to evaluate the qualitative impact of food on human health.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery

UniBE Contributor:

Laederach, Kurt

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

1873-4847

Publisher:

Elsevier

Language:

English

Submitter:

Kurt Laederach

Date Deposited:

03 Apr 2018 17:34

Last Modified:

03 Apr 2018 17:34

Publisher DOI:

10.1016/j.jnutbio.2017.02.012

PubMed ID:

28319853

Additional Information:

Die Hauptautoren sind im Departement des Inneren, Agroskop Liebefeld/ Posieux tätig und nicht in der Universität Bern zu finden

Uncontrolled Keywords:

Dose–Response Gene expression High-fat meal Insulin Oxidative phosphorylation

BORIS DOI:

10.7892/boris.102017

URI:

https://boris.unibe.ch/id/eprint/102017

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