Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid.

Schneider, Christoph; Illi, Marlies; Lötscher, Marius; Wehrli, Marc; von Gunten, Stephan (2017). Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid. Methods in molecular biology, 1643, pp. 23-31. Springer 10.1007/978-1-4939-7180-0_3

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Different protocols are required for the collection and isolation of antibodies from various body sites. For the sample collection factors to be considered include anatomic or physiological particularities. Secretory fluids such as saliva, gastrointestinal fluid, or breast milk may contain degrading enzymes that potentially affect the integrity of isolated antibodies. While the isolation of IgG from plasma is a common and often-described procedure, here we focus on methodological approaches to isolate antibodies immunoglobulin A (IgA) or IgM from plasma or secretory fluids. These protocols shall facilitate research on natural and induced antibodies.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Schneider, Christoph; Wehrli, Marc and von Gunten, Stephan

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1940-6029

Publisher:

Springer

Language:

English

Submitter:

Jana Berger

Date Deposited:

20 Oct 2017 09:01

Last Modified:

14 Jun 2018 09:40

Publisher DOI:

10.1007/978-1-4939-7180-0_3

PubMed ID:

28667527

Uncontrolled Keywords:

Antibody collection Antibody isolation Breast milk Gastrointestinal fluid Humoral immunity IgA IgM Saliva Secretory antibodies

BORIS DOI:

10.7892/boris.102436

URI:

https://boris.unibe.ch/id/eprint/102436

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