Bioengineering a Human Face Graft: The Matrix of Identity.

Duisit, Jérôme; Maistriaux, Louis; Taddeo, Adriano; Orlando, Giuseppe; Joris, Virginie; Coche, Emmanuel; Behets, Catherine; Lerut, Jan; Dessy, Chantal; Cossu, Giulio; Vögelin, Esther; Rieben, Robert; Gianello, Pierre; Lengelé, Benoît (2017). Bioengineering a Human Face Graft: The Matrix of Identity. Annals of surgery, 266(5), pp. 754-764. Lippincott Williams & Wilkins 10.1097/SLA.0000000000002396

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OBJECTIVE During the last decade, face allotransplantation has been shown to be a revolutionary reconstructive procedure for severe disfigurements. However, offer to patients remains limited due to lifelong immunosuppression. To move forward in the field, a new pathway in tissue engineering is proposed. BACKGROUND Our previously reported technique of matrix production of a porcine auricular subunit graft has been translated to a human face model. METHODS 5 partial and 1 total face grafts were procured from human fresh cadavers. After arterial cannulation, the specimens were perfused using a combined detergent/polar solvent decellularization protocol. Preservation of vascular patency was assessed by imaging, cell and antigen removal by DNA quantification and histology. The main extracellular matrix proteins and associated cytokines were evaluated. Lip scaffolds were cultivated with dermal, muscle progenitor and endothelial cells, either on discs or in a bioreactor. RESULTS Decellularization was successful in all facial grafts within 12 days revealing acellular scaffolds with full preservation of innate morphology. Imaging demonstrated a preservation of the entire vascular tree patency. Removal of cells and antigens was confirmed by reduction of DNA and antigen markers negativation. Microscopic evaluation revealed preservation of tissue structures as well as of major proteins. Seeded cells were viable and well distributed within all scaffolds. CONCLUSIONS Complex acellular facial scaffolds were obtained, preserving simultaneously a cell-friendly extracellular matrix and a perfusable vascular tree. This step will enable further engineering of postmortem facial grafts, thereby offering new perspectives in composite tissue allotransplantation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery > Hand Surgery
04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Herz und Gefässe
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Handchirurgie
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Taddeo, Adriano; Vögelin, Esther and Rieben, Robert

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0003-4932

Publisher:

Lippincott Williams & Wilkins

Language:

English

Submitter:

Veronika Picha

Date Deposited:

13 Sep 2017 08:26

Last Modified:

15 Feb 2019 09:07

Publisher DOI:

10.1097/SLA.0000000000002396

PubMed ID:

28742686

BORIS DOI:

10.7892/boris.103428

URI:

https://boris.unibe.ch/id/eprint/103428

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