Laser scanning microscopy combined with image restoration to analyse a 3D model of the human epithelial airway barrier

Rothen-Rutishauser, Barbara; Lehmann, Andrea D; Clift, Martin J D; Blank, Fabian; Gehr, Peter (2010). Laser scanning microscopy combined with image restoration to analyse a 3D model of the human epithelial airway barrier. Swiss medical weekly, 140, w13060. Muttenz: EMH Schweizerischer Ärzteverlag 10.4414/smw.2010.13060

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A laser scanning microscope collects information from a thin, focal plane and ignores out of focus information. During the past few years it has become the standard imaging method to characterise cellular morphology and structures in static as well as in living samples. Laser scanning microscopy combined with digital image restoration is an excellent tool for analysing the cellular cytoarchitecture, expression of specific proteins and interactions of various cell types, thus defining valid criteria for the optimisation of cell culture models. We have used this tool to establish and evaluate a three dimensional model of the human epithelial airway wall.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Rothen-Rutishauser, Barbara; Clift, Martin; Blank, Fabian and Gehr, Peter

ISSN:

1424-7860

Publisher:

EMH Schweizerischer Ärzteverlag

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:09

Last Modified:

06 Dec 2013 13:20

Publisher DOI:

10.4414/smw.2010.13060

PubMed ID:

20458650

Web of Science ID:

000282844400001

URI:

https://boris.unibe.ch/id/eprint/1035 (FactScience: 201731)

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