Distinct Neurotoxicity Profile of Listeriolysin O from Listeria monocytogenes.

Maurer, Jana; Hupp, Sabrina; Bischoff, Carolin; Foertsch, Christina; Mitchell, Timothy J; Chakraborty, Trinad; Iliev, Asparouh Iliev (2017). Distinct Neurotoxicity Profile of Listeriolysin O from Listeria monocytogenes. Toxins, 9(1) MDPI 10.3390/toxins9010034

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Cholesterol-dependent cytolysins (CDCs) are protein toxins that originate from Gram-positive bacteria and contribute substantially to their pathogenicity. CDCs bind membrane cholesterol and build prepores and lytic pores. Some effects of the toxins are observed in non-lytic concentrations. Two pathogens, Streptococcus pneumoniae and Listeria monocytogenes, cause fatal bacterial meningitis, and both produce toxins of the CDC family-pneumolysin and listeriolysin O, respectively. It has been demonstrated that pneumolysin produces dendritic varicosities (dendrite swellings) and dendritic spine collapse in the mouse neocortex, followed by synaptic loss and astrocyte cell shape remodeling without elevated cell death. We utilized primary glial cultures and acute mouse brain slices to examine the neuropathological effects of listeriolysin O and to compare it to pneumolysin with identical hemolytic activity. In cultures, listeriolysin O permeabilized cells slower than pneumolysin did but still initiated non-lytic astrocytic cell shape changes, just as pneumolysin did. In an acute brain slice culture system, listeriolysin O produced dendritic varicosities in an NMDA-dependent manner but failed to cause dendritic spine collapse and cortical astrocyte reorganization. Thus, listeriolysin O demonstrated slower cell permeabilization and milder glial cell remodeling ability than did pneumolysin and lacked dendritic spine collapse capacity but exhibited equivalent dendritic pathology.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Hupp, Sabrina and Iliev, Asparouh Iliev

ISSN:

2072-6651

Publisher:

MDPI

Language:

English

Submitter:

Sabrina Hupp

Date Deposited:

02 Nov 2017 16:17

Last Modified:

11 Sep 2019 15:57

Publisher DOI:

10.3390/toxins9010034

PubMed ID:

28098781

Uncontrolled Keywords:

acute slices dendritic spines listeriolysin O meningitis varicosities

BORIS DOI:

10.7892/boris.105223

URI:

https://boris.unibe.ch/id/eprint/105223

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