The complex-in-a-complex cation [Pt(acac)2⊂(p-cym)6Ru6(tpt)2(dhnq)3]6+: Its stability towards biological ligands

Paul, Lydia; Therrien, bruno; Furrer, Julien (2018). The complex-in-a-complex cation [Pt(acac)2⊂(p-cym)6Ru6(tpt)2(dhnq)3]6+: Its stability towards biological ligands. Inorganica chimica acta, 469(1), pp. 1-10. Elsevier Sequoia 10.1016/j.ica.2017.08.045

[img] Text
1-s2.0-S0020169317312896-main.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

The reactivity of the highly cytotoxic complex-in-a-complex cation [Pt(acac)2⊂(p-cym)6Ru6(tpt)2(dhnq)3]6+ ([Pt(acac)2⊂1]6+) towards amino acids, proteins, nucleotides and an oligonucleotide was investigated by NMR, MS, UV/Vis and CD experiments. The similarities and differences between the metallaprism [Pt(acac)2⊂1]6+, its empty counterpart [1]6+ and the previously reported smaller complex-in-a-complex cation [Pt(acac)2⊂(p-cym)6Ru6(tpt)2(dhbq)3]6+ ([Pt(acac)2⊂2]6+) were described. The reactivity of [Pt(acac)2⊂1]6+ towards amino acids and the degradation products formed were similar to those observed with [1]6+ and [Pt(acac)2⊂2]6+ but the kinetic of the reactions was much slower. Interestingly, [Pt(acac)2⊂1]6+ imposed stronger structural effects on the human proteins HsA, Tf, Mb, Cyt c, and Ub compared to the empty metallaprism [1]6+. The effects were similar to those of [Pt(acac)2⊂2]6+. Unlike [Pt(acac)2⊂2]6+ and as observed previously with [1]6+, [Pt(acac)2⊂1]6+ did not react with nucleotides. However, similar interactions with a double stranded oligonucleotide were observed in the presence of both metallaprisms [Pt(acac)2⊂1]6+ and [1]6+. In solution, [Pt(acac)2⊂1]6+ disassembles at a lower rate than [Pt(acac)2⊂2]6+. Overall, the results show that the enhanced stability of [1]6+ is kept when a guest is trapped inside the hydrophobic cavity, suggesting that the half-life of [Pt(acac)2⊂1]6+ in the blood is probably sufficiently long to enter cells and reach the target intact.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Paul, Lydia and Furrer, Julien

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0020-1693

Publisher:

Elsevier Sequoia

Language:

English

Submitter:

Julien Henri Lucien Furrer

Date Deposited:

29 Nov 2017 13:14

Last Modified:

29 Nov 2017 13:18

Publisher DOI:

10.1016/j.ica.2017.08.045

BORIS DOI:

10.7892/boris.105244

URI:

https://boris.unibe.ch/id/eprint/105244

Actions (login required)

Edit item Edit item
Provide Feedback