Changes in CXCL12/CXCR4-chemokine expression during onset of colorectal malignancies

Oliveira Frick, Vilma; Rubie, Claudia; Ghadjar, Pirus; Faust, Sabrina K; Wagner, Mathias; Gräber, Stefan; Schilling, Martin K (2011). Changes in CXCL12/CXCR4-chemokine expression during onset of colorectal malignancies. Tumor biology, 32(1), pp. 189-96. Dordrecht: Springer 10.1007/s13277-010-0112-y

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Chemokines have been proposed to contribute to tumour growth and metastatic spread of several cancer entities. Here, we examined the relative levels of CXCL12/CXCR4 in resection specimens from patients with different malignant and non-malignant colorectal diseases as well as colorectal liver metastases (CRLM). CXCL12/CXCR4 mRNA and protein expression profiles were assessed by quantitative real-time PCR, Western blot analysis, enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry in resection specimens from patients with ulcerative colitis (UC; n = 15), colorectal adenoma (CRA; n = 15), colorectal adenocarcinoma (CRC; n = 47) and CRLM (n = 16). Corresponding non-affected tissues served as control. In contrast to UC tissues, CXCL12 showed a distinct down-regulation in CRA, CRC and CRLM specimens, whereas the corresponding receptor CXCR4 demonstrated a significant up-regulation in CRC and CRLM related to corresponding non-affected tissues (p < 0.05, respectively). Our results strongly suggest an association between CXCL12/CXCR4 expression and the induction of CRA, CRC and the development of CRLM. Therefore, CXCR4 may be a potential target for specific therapeutic interventions.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Radiation Oncology

UniBE Contributor:

Ghadjar, Pirus

ISSN:

1010-4283

Publisher:

Springer

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:09

Last Modified:

16 Jul 2018 14:36

Publisher DOI:

10.1007/s13277-010-0112-y

PubMed ID:

20865359

Web of Science ID:

000285469700020

URI:

https://boris.unibe.ch/id/eprint/1054 (FactScience: 201776)

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