Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes.

Zuppinger, Christian; Gibbons, George; Dutta-Passecker, Priyanka; Segiser, Adrian; Most, Henriette; Suter, Thomas M (2017). Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes. European journal of histochemistry, 61(2), p. 2763. PAGEPress 10.4081/ejh.2017.2763

[img]
Preview
Text
ejh-61-2-2763.pdf - Published Version
Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC).

Download (828kB) | Preview

Recent innovations in stem cell technologies and the availability of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have opened new possibilities for studies and drug testing on human cardiomyocytes in vitro. Still, there are concerns about the precise nature of such 'reprogrammed' cells. We have performed an investigation using immunocytochemistry and confocal microscopy on several cellular features using commercially available hiPSC-CMs. For some selected developmentally regulated or cardiac chamber-specific proteins, we have compared the results from hiPSC-derived cardiomyocytes with freshly isolated, ventricular cardiomyocytes from adult rats. The results show that all typical cardiac proteins are expressed in these hiPSC-CMs. Furthermore, intercalated disc-like structures, calcium cycling proteins, and myofibrils are present. However, some of these proteins are only known from early developmental stages of the ventricular myocardium or the diseased adult heart. A heterogeneous expression pattern in the cell population was noted for some muscle proteins, such as for myosin light chains, or incomplete organization in sarcomeres, such as for telethonin. These observations indicate that hiPSC-CMs can be considered genuine human cardiomyocytes of an early developmental state. The here described marker proteins of maturation may become instrumental in future studies attempting the improvement of cardiomyocyte in vitro models.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiovascular Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiology

UniBE Contributor:

Zuppinger, Christian; Segiser, Adrian and Most, Henriette

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2038-8306

Publisher:

PAGEPress

Language:

English

Submitter:

Daniela Huber

Date Deposited:

20 Sep 2017 16:47

Last Modified:

24 Sep 2017 02:17

Publisher DOI:

10.4081/ejh.2017.2763

PubMed ID:

28735524

BORIS DOI:

10.7892/boris.105427

URI:

https://boris.unibe.ch/id/eprint/105427

Actions (login required)

Edit item Edit item
Provide Feedback