Macrococcus canis sp. nov., a skin bacterium associated with infections in dogs

Gobeli, Stefanie; Cotting, Kerstin; Gomez Sanz, Elena; Collaud, Alexandra; Thomann, Andreas; Brodard, Isabelle; Rodriguez, Sabrina; Strauss, Christian; Perreten, Vincent (2017). Macrococcus canis sp. nov., a skin bacterium associated with infections in dogs. International journal of systematic and evolutionary microbiology, 67(3), pp. 621-626. Society for General Microbiology SGM 10.1099/ijsem.0.001673

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Gram-stain-positive cocci were isolated from miscellaneous sites of the skin of healthy dogs as well as from infection sites in dogs. The closest relative by sequencing of the 16S rRNA gene was Macrococcus caseolyticus with 99.7% sequence identity, but compared with M. caseolyticus, the novel strains shared only 90.8 to 93.5%DNA sequence identity with cpn60, dnaJ, rpoB and sodA partial genes, respectively. The novel strains also exhibited differential phenotypic characteristics from M. caseolyticus, and the majority displayed a visible haemolysis on sheep blood agar, while M. caseolyticus did not have any haemolytic activity. They generated different matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS spectral profiles compared with the other species of the genus Macrococcus. Furthermore, strain KM 45013T shared only 53.7% DNA–DNA relatedness with the type strain of M. caseolyticus, confirming that they do not belong to the same species. The DNA G+C content of strain KM 45013T was 36.9 mol%. The most abundant fatty acids were C14 : 0, C18 : 3!6c (6, 9, 12) and C16 : 0 n alcohol. MK-6 was the menaquinone type of KM 45013T. Cell-wall structure analysis revealed that the peptidoglycan type was A3a L-Lys–Gly2–L-Ser. Based on genotypic and chemotaxonomic characteristics, we propose to classify these strains within a novel species of the genus Macrococcus for which the name Macrococcus canis sp. nov. is proposed. The type strain is KM 45013T (=DSM 101690T=CCOS 969T=CCUG 68920T).

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Other Institutions > Teaching Staff, Vetsuisse Faculty
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Gobeli, Stefanie; Cotting, Kerstin; Gomez Sanz, Elena; Collaud, Alexandra; Thomann, Andreas; Brodard, Isabelle; Rodriguez, Sabrina; Strauss, Christian and Perreten, Vincent

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1466-5026

Publisher:

Society for General Microbiology SGM

Language:

English

Submitter:

Sabrina Rodriguez Campos

Date Deposited:

21 Nov 2017 08:08

Last Modified:

21 Nov 2017 13:49

Publisher DOI:

10.1099/ijsem.0.001673

PubMed ID:

27902286

BORIS DOI:

10.7892/boris.105654

URI:

https://boris.unibe.ch/id/eprint/105654

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