RNA/DNA co-analysis from bloodstains on aged polyvinyl-alcohol gloves prepared for securing evidence from the hands of victims of fatal gunshot injuries.

Grabmüller, Melanie; Courts, Cornelius; Madea, Burkhard; Eichhorst, Tim; Schyma, Christian (2018). RNA/DNA co-analysis from bloodstains on aged polyvinyl-alcohol gloves prepared for securing evidence from the hands of victims of fatal gunshot injuries. International journal of legal medicine, 132(1), pp. 53-66. Springer 10.1007/s00414-017-1687-2

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In contrast to cumulative techniques (e.g., tape-lift) for qualitative gunshot residues (GSR) analysis, topographic methods are commonly applied to preserve the integrity of evidence from a shooter's or victim's hand in cases of gun-related crimes. Topographic sampling techniques employing adhesive foils, latex, or the polyvinyl alcohol (PVAL) method enable unambiguous sampling of biological and non-biological trace material while preserving its spatial distribution and relation to each other. The PVAL method in particular allows for a topographically veridic and quantitative conservation of traces of GSR and biological stains that are embedded in the PVAL glove, because it completely removes these traces from the hand. The present study investigated the success rates of STR profiling and the detection of blood and brain-specific gene expression from minimal traces of blood splatter as well as parallel to the positive detection of gunshot residues embedded in 17 PVAL gloves taken from the hands of deceased persons in the context of homicide cases in the period between 1996 and 2003. The water-soluble PVAL matrix is shown to be fully compatible with successful STR profiling and the detection of blood- and brain-specific miRNA expression, even after up to 20 years of storage, demonstrating that this sampling technique offers advantages compared to other more simplistic sampling methods like taping.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Legal Medicine
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Medicine

UniBE Contributor:

Schyma, Christian

ISSN:

0937-9827

Publisher:

Springer

Language:

English

Submitter:

Antoinette Angehrn

Date Deposited:

17 Oct 2017 09:58

Last Modified:

25 Apr 2018 12:20

Publisher DOI:

10.1007/s00414-017-1687-2

PubMed ID:

28956148

Uncontrolled Keywords:

Blood- and brain-specific expression Bloodstains Gunshot residues PVAL Polyvinyl alcohol method RNA/DNA co-analysis

BORIS DOI:

10.7892/boris.106093

URI:

https://boris.unibe.ch/id/eprint/106093

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