Lung responses in murine models of experimental asthma: Value of house dust mite over ovalbumin sensitization.

Doras, Camille; Petak, Ferenc; Bayat, Sam; Baudat, Aurélie; von Garnier, Christophe; Eigenmann, Philippe; Habre, Walid (2018). Lung responses in murine models of experimental asthma: Value of house dust mite over ovalbumin sensitization. Respiratory physiology & neurobiology, 247, pp. 43-51. Elsevier 10.1016/j.resp.2017.09.001

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Ovalbumin (OVA) sensitization has limitations in modelling asthma. Thus, we examined the value of allergic sensitization using a purified natural allergen, house dust mite (HDM), over the sensitization performed with OVA. Mice were sham-treated, or sensitized with OVA- or HDM with identical chronology. Airway resistance, tissue damping and elastance were assessed under control conditions and after challenging the animals with methacholine (MCh) and the specific allergen. Inflammatory profile of the bronchoalveolar lavage fluid was characterized and lung histology was performed. While no difference in the lung responsiveness to the specific allergen was noted, hyperresponsiveness to MCh was observed only in the HDM-sensitized animals in the lung peripheral parameters. Lung inflammation differed between the models, but excessive bronchial smooth muscle remodelling occurred only with OVA. In conclusion, we demonstrate that a purified natural allergen offers a more relevant murine model of human allergic asthma by expressing the key features of this chronic inflammatory disease both in the lung function and structure.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)

UniBE Contributor:

von Garnier, Christophe

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1569-9048

Publisher:

Elsevier

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

27 Nov 2017 13:37

Last Modified:

13 Feb 2018 15:00

Publisher DOI:

10.1016/j.resp.2017.09.001

PubMed ID:

28890402

Uncontrolled Keywords:

Airway hyperresponsiveness Allergen exposure Animal models Asthma Mice

BORIS DOI:

10.7892/boris.106602

URI:

https://boris.unibe.ch/id/eprint/106602

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