On the micro-indentation of plant cells in a tissue context

Mosca, Gabriella; Sapala, Aleksandra; Strauss, Soeren; Routier-Kierzkowska, Anne-Lise; Smith, Richard S. (2017). On the micro-indentation of plant cells in a tissue context. Physical Biology, 14(1), 015003. IOP Publishing 10.1088/1478-3975/aa5698

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The effect of geometry on cell stiffness measured with micro-indentation techniques has been explored in single cells, however it is unclear if results on single cells can be readily transferred to indentation experiments performed on a tissue in vivo . Here we explored this question by using simulation models of osmotic treatments and micro-indentation experiments on 3D multicellular tissues with the finite element method. We found that the cellular context does affect measured cell stiffness, and that several cells of context in each direction are required for optimal results. We applied the model to micro-indentation data obtained with cellular force microscopy on the sepal of A. thaliana , and found that differences in measured stiffness could be explained by cellular geometry, and do not necessarily indicate differences in cell wall material properties or turgor pressure.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Mosca, Gabriella; Routier, Anne-Lise and Smith, Richard Simon

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

1478-3975

Publisher:

IOP Publishing

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

06 Dec 2017 12:30

Last Modified:

06 Dec 2017 12:30

Publisher DOI:

10.1088/1478-3975/aa5698

PubMed ID:

28181475

BORIS DOI:

10.7892/boris.106876

URI:

https://boris.unibe.ch/id/eprint/106876

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