A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef

Kamies, Rizqah; Farrant, Jill M.; Tadele, Zerihun; Cannarozzi, Gina; Rafudeen, Mohammed Suhail (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes, 5(4) MDPI 10.3390/proteomes5040032

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The orphan crop, Eragrostis tef, was subjected to controlled drought conditions to observe the physiological parameters and proteins changing in response to dehydration stress. Physiological measurements involving electrolyte leakage, chlorophyll fluorescence and ultra-structural analysis showed tef plants tolerated water loss to 50% relative water content (RWC) before adverse effects in leaf tissues were observed. Proteomic analysis using isobaric tag for relative and absolute quantification (iTRAQ) mass spectrometry and appropriate database searching enabled the detection of 5727 proteins, of which 211 proteins, including a number of spliced variants, were found to be differentially regulated with the imposed stress conditions. Validation of the iTRAQ dataset was done with selected stress-related proteins, fructose-bisphosphate aldolase (FBA) and the protective antioxidant proteins, monodehydroascorbate reductase (MDHAR) and peroxidase (POX). Western blot analyses confirmed protein presence and showed increased protein abundance levels during water deficit while enzymatic activity for FBA, MDHAR and POX increased at selected RWC points. Gene ontology (GO)-term enrichment and analysis revealed terms involved in biotic and abiotic stress response, signaling, transport, cellular homeostasis and pentose metabolic processes, to be enriched in tef upregulated proteins, while terms linked to reactive oxygen species (ROS)-producing processes under water-deficit, such as photosynthesis and associated light harvesting reactions, manganese transport and homeostasis, the synthesis of sugars and cell wall catabolism and modification, to be enriched in tef downregulated proteins.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Tadele, Zerihun and Cannarozzi, Gina Michelle

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

2227-7382

Publisher:

MDPI

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

12 Dec 2017 15:47

Last Modified:

29 Oct 2019 05:47

Publisher DOI:

10.3390/proteomes5040032

PubMed ID:

29140297

Uncontrolled Keywords:

tef; drought-stress; functional enrichment analysis; GO-term; iTRAQ; physiological characterisation; quantitative proteomics; stress-responsive proteins

BORIS DOI:

10.7892/boris.107106

URI:

https://boris.unibe.ch/id/eprint/107106

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