Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development.

Saini, Ekta; Zeeshan, Mohammad; Brady, Declan; Pandey, Rajan; Kaiser, Gesine; Koreny, Ludek; Kumar, Pradeep; Thakur, Vandana; Tatiya, Shreyansh; Katris, Nicholas J; Limenitakis Stanway, Rebecca; Kaur, Inderjeet; Green, Judith L; Bottrill, Andrew R; Guttery, David S; Waller, Ross F; Heussler, Volker; Holder, Anthony A; Mohmmed, Asif; Malhotra, Pawan; ... (2017). Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development. Scientific Reports, 7(1), p. 15577. Nature Publishing Group 10.1038/s41598-017-15781-z

[img]
Preview
Text
Sinai Phil1 2017 Scientific reports.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

Plasmodium parasites, the causative agents of malaria, possess a distinctive membranous structure of flattened alveolar vesicles supported by a proteinaceous network, and referred to as the inner membrane complex (IMC). The IMC has a role in actomyosin-mediated motility and host cell invasion. Here, we examine the location, protein interactome and function of PhIL1, an IMC-associated protein on the motile and invasive stages of both human and rodent parasites. We show that PhIL1 is located in the IMC in all three invasive (merozoite, ookinete-, and sporozoite) stages of development, as well as in the male gametocyte and locates both at the apical and basal ends of ookinete and sporozoite stages. Proteins interacting with PhIL1 were identified, showing that PhIL1 was bound to only some proteins present in the glideosome motor complex (GAP50, GAPM1-3) of both P. falciparum and P. berghei. Analysis of PhIL1 function using gene targeting approaches indicated that the protein is required for both asexual and sexual stages of development. In conclusion, we show that PhIL1 is required for development of all zoite stages of Plasmodium and it is part of a novel protein complex with an overall composition overlapping with but different to that of the glideosome.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria
08 Faculty of Science > Department of Biology > Institute of Cell Biology
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Kaiser, Gesine; Limenitakis Stanway, Rebecca and Heussler, Volker

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2045-2322

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Volker Heussler

Date Deposited:

01 Dec 2017 13:43

Last Modified:

23 Oct 2019 19:33

Publisher DOI:

10.1038/s41598-017-15781-z

PubMed ID:

29138437

BORIS DOI:

10.7892/boris.107202

URI:

https://boris.unibe.ch/id/eprint/107202

Actions (login required)

Edit item Edit item
Provide Feedback