Novel β-Lactamase blaARL in Staphylococcus arlettae.

Andreis, Sabrina Nathalie; Perreten, Vincent; Schwendener, Sybille (2017). Novel β-Lactamase blaARL in Staphylococcus arlettae. mSphere, 2(3), e00117-17. American Society for Microbiology 10.1128/mSphere.00117-17

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Whole-genome sequencing of penicillin-resistant Staphylococcus arlettae strain SAN1670 from bovine mastitis milk revealed a novel β-lactamase operon consisting of the β-lactamase-encoding gene blaARL, the antirepressor-encoding gene blaR1ARL, and the repressor-encoding gene blaIARL. The functionality of blaARL was demonstrated by gene expression in Staphylococcus aureus. The blaARL operon was chromosomally located in SAN1670 and present in 10 additional unrelated strains, suggesting intrinsic penicillin resistance in S. arlettae. Furthermore, a GenBank search revealed more unique potential β-lactamases in Staphylococcus species. IMPORTANCE Penicillins are an important group of antibiotics used to treat various types of infections caused by Gram-positive bacteria. So far, the blaZ gene was the only known β-lactamase gene in staphylococci. However, other putative β-lactamases were identified, and one of them was shown to be a novel functional β-lactamase encoded by blaARL in Staphylococcus arlettae, further limiting treatment options.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Andreis, Sabrina Nathalie; Perreten, Vincent and Schwendener, Sybille

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

2379-5042

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

10 Apr 2018 09:05

Last Modified:

10 Apr 2018 09:13

Publisher DOI:

10.1128/mSphere.00117-17

PubMed ID:

28497118

Uncontrolled Keywords:

antibiotic resistance beta-lactamases coagulase-negative staphylococci penicillinase

BORIS DOI:

10.7892/boris.107227

URI:

https://boris.unibe.ch/id/eprint/107227

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