Intracellular localization of serine acetyltransferase in spinach leaves

Brunold, Christian; Suter, Marianne (1982). Intracellular localization of serine acetyltransferase in spinach leaves. Planta, 155(4), pp. 321-327. Springer 10.1007/BF00429459

[img] Text
1982_Planta_155_321.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (677kB) | Request a copy

Intact chloroplasts isolated from spinach leaves by a combination of differential and Percoll density gradient centrifugation and free of mitochondrial and peroxisomal contamination contained about 35% of the total leaf serine acetyltransferase (EC 2.3.1.30) activity. No appreciable activity of the enzyme could be detected in the gradient fractions containing broken chloroplasts, mitochondria, and peroxisomes. L-cysteine added to the incubation mixture at 1 mM almost completely inhibited serine acetyltransferase activity, both of leaf and chloroplast extracts. D-cysteine was much less inhibitory. L-cystine up to 5 mM and O-acetyl-L-serine up to 10 mM had no effect on the enzyme activity. When measured at pH 8.4, the enzyme extracted from the leaves had a K m for L-serine of 2.4, the enzyme from the chloroplasts a K m of 2.8 mM.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Brunold, Christian and Suter, Marianne

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0935

Publisher:

Springer

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

13 Jun 2018 18:01

Last Modified:

22 Oct 2019 21:32

Publisher DOI:

10.1007/BF00429459

Uncontrolled Keywords:

chloroplast (serine acetyltransferase); cysteine; serine acetyltransferase; spinacia

BORIS DOI:

10.7892/boris.107346

URI:

https://boris.unibe.ch/id/eprint/107346

Actions (login required)

Edit item Edit item
Provide Feedback