Properties and regulation of adenosine 5′-phosphosulfate sulfotransferase from suspension cultures of Nicotiana sylvestris

Jenni, B. E.; Brunold, Christian; Zrÿd, J. -P.; Lavanchy, P. (1980). Properties and regulation of adenosine 5′-phosphosulfate sulfotransferase from suspension cultures of Nicotiana sylvestris. Planta, 150(2), pp. 140-143. Springer 10.1007/BF00582357

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The properties and the regulation of adenosine 5′-phosphosulfate sulfotransferase extracted from cell suspension cultures ofNicotiana sylvestris was investigated. Optimal adenosine 5′-phosphosulfate sulfotransferase activity was obtained from the cells by extraction with 0.1 M tris-HCl, pH8.0, containing 2 M MgSO4 and 10 mM dithioerythritol. The K m for adenosine 5′-phosphosulfate in the sulfotransferase reaction was about 11 μM. Adenosine 5′-phosphosulfate in concentrations above 50 μM were inhibitory. The extratable adenosine 5′-phosphosulfate sulfotransferase activity decreased during cultivation with sulfate as the sole sulfur source, but after about 3 days it reached a constant level (50 to 100 nmol activated sulfate transferred h-1 mg-1 protein) which was maintained for at least 24 h. Addition of 0.5 mM cysteine to the culture medium decreased the extractable adenosine 5′-phosphosulfate sulfotransferase activity and blocked growth completely. With 0.1 mM cysteine an enzyme level of about 10% of the initial value was reached within 6 to 12 h without significant inhibition of growth. The added cysteine was absorbed rapidly and after 24 h cysteine could no longer be detected in the medium. Before the cysteine was completely depleted, the activity of adenosine 5′-phosphosulfate sulfotransferase started to increase, reaching ultimately a level which was comparable to the initial value.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Brunold, Christian

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0935

Publisher:

Springer

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

13 Jun 2018 18:02

Last Modified:

06 Nov 2019 19:04

Publisher DOI:

10.1007/BF00582357

Uncontrolled Keywords:

adenosine 5′-phosphosulfate sulfotransferase; cell suspension culture; cysteine; enzyme regulation; Nicotiana

BORIS DOI:

10.7892/boris.107347

URI:

https://boris.unibe.ch/id/eprint/107347

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