Localization of O-acetyl-L-serine sulfhydrylase in Spinacia oleracea L.

Fankhauser, Heinz; Brunold, Christian (1979). Localization of O-acetyl-L-serine sulfhydrylase in Spinacia oleracea L. Plant Science Letters, 14(2), pp. 185-192. Elsevier 10.1016/0304-4211(79)90058-0

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Roots and hypocotyls of 11 day-old spinach seedlings with fully developed cotyledons contained 30–35% of the total O-acetyl-L-serine sulfhydrylase (OASSase) extractable from the plants. After centrifugation of homogenates from roots on sucrose density gradients, OASSase activity was present in the fractions containing the proplastids. In leaf extract two forms of OASSase could be detected by polyacrylamide disc gel electrophoresis. The form with the lower electrophoretic mobility in 0.1 M Tris-glycine at pH 8.4 could be attributed to the chloroplasts. The chloroplast enzyme was less stable than the extrachloroplastic form if stored without dithioerythritol (DTE).

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Brunold, Christian

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0304-4211

Publisher:

Elsevier

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

13 Jun 2018 17:53

Last Modified:

21 Nov 2019 00:33

Publisher DOI:

10.1016/0304-4211(79)90058-0

BORIS DOI:

10.7892/boris.107358

URI:

https://boris.unibe.ch/id/eprint/107358

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