Effect of high and low sulfate concentrations on adenosine 5'-phosphosulfate sulfotransferase activity from Lemna minor

Brunold, Christian; Suter, Marianne; Lavanchy, Pierre (1987). Effect of high and low sulfate concentrations on adenosine 5'-phosphosulfate sulfotransferase activity from Lemna minor. Physiologia Plantarum, 70(2), pp. 168-174. Blackwell 10.1111/j.1399-3054.1987.tb06127.x

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The effect of Na2SO4 concentrations from 0 to 17.6 mM in the nutrient solution of Lemna minor L. strain 6580 on adenosine 5′-phosphosulfate sulfotransferase activity was examined. Routinely, the plants were cultivated on 0.88 mA SO42−. The enzyme activity was increased by 50 to 100% after transfer to 0 or 0.0088 mM SO42−. Transfer back to 0.88 mM rapidly decreased the enzyme activity to the initial level. Cultivation on 17.6 mM Na2SO4 redueed extractable adenosine 5′-phosphosulfate sulfotransferase by 50%. The original level was rapidly re-established on 0,88 mM. In control experiments, a decrease in adenosine 5′-phosphosulfate sulfotransferase activity was also induced by K2 SO4, whereas NaCl caused a small increase. This indicates that the observed effects are dependent on the sulfate ion. ATP-sulfurylase activity measured for comparison was only significantly affected by the omission of sulfate, which induced a 20% increase, indicating that this enzyme activity from Lemna minor is less suseeptible to changes in medium sulfate than adenosine 5′-phosphosulfate sulfotransferase. A close relationship between adenosine 5′-phosphosulfate sulfotransferase activity and the content of asparagine, glutamine, non-protein thiols and sulfate in the tissue was detected, indicating a positive control mechanism induced by amides and a negative mechanism induced by thiols and sulfate.

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