CLEC5A (MDL-1) is a novel PU.1 transcriptional target during myeloid differentiation

Batliner, Jasmin; Mancarelli, Maria Michela; Jenal, Mathias; Reddy, Venkateshwar A; Fey, Martin F; Torbett, Bruce E; Tschan, Mario P (2011). CLEC5A (MDL-1) is a novel PU.1 transcriptional target during myeloid differentiation. Molecular immunology, 48(4), pp. 714-9. Amsterdam: Elsevier 10.1016/j.molimm.2010.10.016

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C-type lectin domain family 5, member A (CLEC5A), also known as myeloid DNAX activation protein 12 (DAP12)-associating lectin-1 (MDL-1), is a cell surface receptor strongly associated with the activation and differentiation of myeloid cells. CLEC5A associates with its adaptor protein DAP12 to activate a signaling cascade resulting in activation of downstream kinases in inflammatory responses. Currently, little is known about the transcriptional regulation of CLEC5A. We identified CLEC5A as one of the most highly induced genes in a microarray gene profiling experiment of PU.1 restored myeloid PU.1-null cells. We further report that CLEC5A expression is significantly reduced in several myeloid differentiation models upon PU.1 inhibition during monocyte/macrophage or granulocyte differentiation. In addition, CLEC5A mRNA expression was significantly lower in primary acute myeloid leukemia (AML) patient samples than in macrophages and granulocytes from healthy donors. Moreover, we found activation of a CLEC5A promoter reporter by PU.1 as well as in vivo binding of PU.1 to the CLEC5A promoter. Our findings indicate that CLEC5A expression in monocyte/macrophage and granulocytes is regulated by PU.1.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology

UniBE Contributor:

Fey, Martin and Tschan, Mario

ISSN:

0161-5890

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:09

Last Modified:

06 Dec 2013 13:20

Publisher DOI:

10.1016/j.molimm.2010.10.016

PubMed ID:

21094529

Web of Science ID:

000286955400041

URI:

https://boris.unibe.ch/id/eprint/1074 (FactScience: 201821)

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