Tomographic in vivo microscopy for the study of lung physiology at the alveolar level

Lovric, Goran; Mokso, Rajmund; Arcadu, Filippo; Vogiatzis Oikonomidis, Ioannis; Schittny, Johannes; Roth-Kleiner, Matthias; Stampanoni, Marco (2017). Tomographic in vivo microscopy for the study of lung physiology at the alveolar level. Scientific Reports, 7(1), p. 12545. Nature Publishing Group 10.1038/s41598-017-12886-3

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Lungs represent the essential part of the mammalian respiratory system, which is reflected in the fact that lung failure still is one of the leading causes of morbidity and mortality worldwide. Establishing the connection between macroscopic observations of inspiration and expiration and the processes taking place at the microscopic scale remains crucial to understand fundamental physiological and pathological processes. Here we demonstrate for the first time in vivo synchrotron-based tomographic imaging of lungs with pixel sizes down to a micrometer, enabling first insights into high-resolution lung structure. We report the methodological ability to study lung inflation patterns at the alveolar scale and its potential in resolving still open questions in lung physiology. As a first application, we identified heterogeneous distension patterns at the alveolar level and assessed first comparisons of lungs between the in vivo and immediate post mortem states.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Vogiatzis Oikonomidis, Ioannis and Schittny, Johannes

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2045-2322

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Johannes Schittny

Date Deposited:

11 Dec 2017 12:35

Last Modified:

17 Dec 2017 02:17

Publisher DOI:

10.1038/s41598-017-12886-3

PubMed ID:

28970505

BORIS DOI:

10.7892/boris.107422

URI:

https://boris.unibe.ch/id/eprint/107422

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